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MiddleBrook Pharmaceuticals
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National Reference Center for Legionella
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Journal: Journal of Clinical Microbiology
Article Title: Rapid Microarray-Based Detection of Rifampin, Isoniazid, and Fluoroquinolone Resistance in Mycobacterium tuberculosis by Use of a Single Cartridge
doi: 10.1128/JCM.01249-17
Figure Lengend Snippet: Specificity of the melting curve assay. The x axis shows the mycobacterial species tested, including M. tuberculosis , M. bovis BCG, and nontuberculous mycobacteria. A probe specific for MTBC strains was used as an internal process control for verification of species specificity. Hybridization signals of >0.05 at 63°C are defined as positive and indicate the presence of strains belonging to the MTBC; thus, M. tuberculosis and M. bovis BCG were measured to be positive at the specific spot. NTM showed no cross-reaction with 106 copies per reaction in a reaction mix (hybridization signal < 0.05).
Article Snippet: The
Techniques: Control, Hybridization